• Ding Qi, Hongmei Li, Shuoqi Wang, Shimeng Wang, Rui Zheng, Ning Liu, Buwei Han, and Li Liu.
• Heilongjiang University of Traditional Chinese Medicine, Heilongjiang, China.
• Medicine (Baltimore). 2022 Dec 2; 101 (48): e31928e31928.

BackgroundThis study aimed to construct an endogenous competition network for cervical squamous intraepithelial lesions using differential gene screening.MethodsGSE149763 was used to screen differentially expressed long non-coding RNAs (lncRNAs) and mRNAs to predict correlated microRNAs (miRNAs). The correlated miRNAs and GSE105409 were used to screen differentially expressed miRNAs for differential co-expression analysis, and the co-expressed differentially expressed miRNAs were used to predict correlated mRNAs. Differentially expressed mRNAs, miRNAs, and lncRNAs were visualized, and differential gene screening, enrichment, and pathway analysis were performed.ResultsThe ceRNA network of cervical squamous intraepithelial was successfully established and a potential differentially expressed network was identified. The key genes were VEGFA and FOS, and the key pathway was the MAPK signaling pathway.ConclusionsThe differential expression and potential effects of the lncRNA BACH1-IT1/miR-140-5p/VEGFA axis, key genes, VEGFA and FOS, and MAPK signaling in CIN were clarified, and the occurrence and potential effects of CIN were further clarified. The underlying molecular mechanism provides a certain degree of reference for subsequent treatments and experimental research.Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc.

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