• İlkay Çinar, Muhammed Bozoğlan, Kürşad Aytekin, Deniz Esenyel, and Cem Zeki Esenyel.
• From the Department of Pathology (Çinar) and Department of Orthopaedic Surgery (Aytekin, Esenyel C), Faculty of Medicine, Giresun University, Giresun; From the Department of Orthopaedic Surgery (Bozoğlan), İzmir Health Sciences University Tepecik Training and Research Hospital, İzmir; Department of Plastic and Reconstructive Surgery (Esenyel D), Kartal Dr. Lütfi Kırdar Training and Research Hospital, İstanbul, Turkey.
• Saudi Med J. 2023 Apr 1; 44 (4): 355362355-362.

ObjectivesTo histopathologically examine the change in gastrocnemius muscle created by sleep disorder in rats.MethodsThis study was carried out at Giresun University, Turkey from December 2018 to January 2021. A total of 30 Wistar rats were separated into 3 groups as the control group (CG), absence of rapid eye movement (REM) sleep (ARS) group, chronic absence of sleep (CAS) group. The lack of sleep was created in all rats. At the end of 21 days, all the rats were euthanized. Degeneration and regeneration findings, and expressions of muscle RING finger 1 (MuRF1), muscle atrophy F-box (MAFbx), tumor necrosis factor (TNF), cyclooxygenase 2 (COX 2), insulin-like growth factor 1 (IGF1) in the gastrocnemius muscles were evaluated histopathologically and immunohistochemically.ResultsDegeneration was found to be greater in the ARS and CAS groups compared to the CG. Regeneration was determined to be significantly lower in the CAS group compared to the ARS group and control group. The number of atrophic fibres was greater in the CAS and ARS groups than in the control group. The IGF1 staining in the CAS group was found to be stronger than in the other 2 groups.ConclusionThis study demonstrated an increase in findings of degeneration in the gastrocnemius muscle of rats with a lack of sleep. The regeneration was reduced in the group with chronic lack of sleep.Copyright: © Saudi Medical Journal.

20 keywords...

hide…