• Neuroscience · Jun 2023

    Limb remote ischemic postconditioning improves glymphatic dysfunction after cerebral ischemia-reperfusion injury.

    • Xiaohong Li, Xiaoli Tan, Qian Zhou, Zhuoxi Xie, Weiting Meng, Yeyu Pang, Lizhen Huang, Zhihao Ding, Yuanhong Hu, Ruhua Li, Guilan Huang, and Hao Li.
    • Department of Neurology, Affiliated Hospital of Guilin Medical University, Guilin 541001, China.
    • Neuroscience. 2023 Jun 15; 521: 203020-30.

    BackgroundDelayed neuronal damage can be caused or aggravated after cerebral ischemia-reperfusion (I/R) injury. Recent studies have shown that glymphatic system dysfunction after cerebral ischemia-reperfusion injury is involved in ischemic brain edema and neuroinflammation, thereby regulating cerebral ischemia-reperfusion injury. The aim of this study is to investigate the changes of glymphatic system after cerebral ischemia-reperfusion injury and whether limb remote ischemic postconditioning (LRIP) can improve the function of glymphatic system to protect the brain.MethodsTo establish a focal brain I/R injury mouse model, this study utilized the middle cerebral artery occlusion/reperfusion (MCAO/R) method. The present study classified eight-week-old C57BL/6 male mice into three groups. The changes in glymphatic function in different periods of ischemia and reperfusion were analyzed through immunofluorescence, transmission electron microscopy (TEM), and Western-Blot (WB) assays. The contents of the evaluation included cerebrospinal fluid flow, swelling degree of brain tissue, aquaporin-4 (AQP4) expression and polarization, and amyloid-β (Aβ) excretion.ResultsIn the early stages of cerebral ischemia, cerebrospinal fluid (CSF) flow is disturbed, accompanied by a decrease in AQP4 polarization. The polarity of AQP4 decreased from 12 h to 72 h of reperfusion, the Aβ deposition. LRIP can increase the expression of β-DG and AQP4 polarization, reduce the deposition of Aβ, improve the function of the glymphatic system, and reduce the expression of AQP4 to play A protective role in brain.ConclusionGlymphatic system impaired after cerebral ischemia-reperfusion injury in mice. LRIP may play a neuroprotective role by improving glymphatic function after I/R.Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.

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