• Patrícia Molz, Diene da Silva Schlickmann, Juliana Priebe Steffens, Eduarda da Silva Limberger Castilhos, Hildegard Hedwig Pohl, Michael Fenech, and FrankeSilvia Isabel RechSIRGraduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do sul, Brazil; Laboratory of Experimental Nutrition, University of Santa Cruz do Sul, Santa Cruz do sul, Brazil. Electronic address: silviafr@uni.
• Graduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do sul, Brazil; Laboratory of Experimental Nutrition, University of Santa Cruz do Sul, Santa Cruz do sul, Brazil; Graduate Program in Biosciences, Federal University of Health Sciences of Porto Alegre School of Medicine, Porto Alegre, Brazil.
• Nutrition. 2023 Sep 1; 113: 112080112080.

ObjectivesLittle is known about the relationship between the supplements used for sport and safety, especially regarding the induction of genotoxicity. Therefore, more knowledge about a DNA damage possibly caused using sport supplements is necessary. The aim of this study was to investigate the potential association between the use of muscle-building supplements and DNA damage in resistance training practitioners.MethodsMuscle-building supplements were classified into three categories based on evidence of efficacy and safety: Strong Evidence to Support Efficacy and Apparently Safe (SESEAS); Limited or Mixed Evidence to Support Efficacy (LMESE), and Little to No Evidence to Support Efficacy and/or Safety (LNESES). DNA damage was evaluated by the comet assay (DNA damage index and frequency) and buccal micronucleus by the cytome assay (micronuclei and nuclear buds). In the sequence, the adjusted analysis of covariance was performed. This study included 307 individuals ages 37.99 ± 13.95 y (52.1% men), of which 157 consumed supplements.ResultsThe results of the comet assay revealed that participants who used supplements had higher DNA damage indexes (P = 0.018) and damage frequency (P = 0.045) than those who reported using no supplements. Moreover, the comet assay also indicated that the participants who used supplements classified into the SESEAS category presented the highest DNA damage index (P = 0.025) and frequency (P = 0.044) compared with those who used no supplements. However, we found no significant difference in the micronuclei and nuclear buds in the evaluated groups (P > 0.05).ConclusionSupplement use is not associated with permanent damage, suggesting that SESEAS supplements are safe for consumption.Copyright © 2023 Elsevier Inc. All rights reserved.

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