• J Coll Physicians Surg Pak · Aug 2023

    Ameliorative Effects of Prolotherapy on Histomorphology of Tibial Articular Cartilage of Chemically Induced Osteoarthritic Knee Joint in a Rat Model.

    • Aqsa Zahid, Khadija Qamar, Asima Tabassum, Mehwish Abaid, Bashir KianiMuhammad RizwanMRDepartment of Anatomy, National University of Medical Sciences, Rawalpindi, Pakistan.Department of Histopathology, National University of Medical Sciences, Rawalpindi, Pakistan., and Maria Aslam.
    • Department of Anatomy, National University of Medical Sciences, Rawalpindi, Pakistan.
    • J Coll Physicians Surg Pak. 2023 Aug 1; 33 (8): 836841836-841.

    ObjectiveTo determine the ameliorative effects of prolotherapy on monosodium iodoacetate (MIA) induced and histomorphological changes in the articular cartilage of tibial condyles at rat knee joint.Study DesignAn experimental study. Place and Duration of the Study: Department of Anatomy, Army Medical College Rawalpindi, NUMS, Rawalpindi, from August to November 2021.MethodologyThirty adult male Sprague Dawley rats were divided into three groups, each having 10 rats. Group A was control. Group B was injected with single dose of 1mg MIA intraarticularly in the right knee to induce osteoarthritic changes. Group C was injected with single dose of 1mg MIA intraarticularly, in right knee was followed by 0.1ml Prolotherapy (3ml of 25% dextrose, 2ml of 2% xylocaine, 1ml of injection neurobion, and 1ml of injection methecobal) as intra articular injection at week 2, 6 and 10 in right knee. Rats were sacrificed after one month of the last dose of Prolotherapy. Articular cartilage was collected for gross and histological examination and compared among the groups.ResultsArticular cartilage belonging to control group A was normal. While group B showed statistically significant deterioration in gross appearance (p = 0.001**), reduction in number of chondrocytes (p = 0.005*) and thickness of articular cartilage (p = 0.001**) in comparison to group A. In group C due to prolotherapy statistically significant improvement in gross appearance (p = 0.034*), increase in number of chondrocytes (p = 0.003*), and thickness of articular cartilage (p = 0.001**) was observed as compared to group B.ConclusionProlotherapy significantly ameliorates histomorphology of tibial articular cartilage against MIA induced osteoarthritic changes in rat knee joint.Key WordsArticular cartilage, Knee joint, Monosodium iodoacetate, Osteoarthritis, Prolotherapy.

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