• Maham Shakoor, Rehma Dar, and Komal Javed.
• Department of Chemical Pathology, University of Health Sciences, Lahore, Pakistan.
• J Coll Physicians Surg Pak. 2023 Nov 1; 33 (11): 128812921288-1292.

ObjectiveTo determine the diagnostic accuracy of serum Presepsin for bacterial sepsis in paediatric patients keeping blood culture as the gold standard test.Study DesignCross-sectional study. Place and Duration of the Study: Department of Chemical Pathology, Paediatric Emergency Department and Intensive Care Unit, King Edward Medical University (KEMU), Mayo Hospital Lahore, from December 2020 to May 2021.MethodologyA total of 57 patients, aged >4 weeks and ≤12 years with suspicion of sepsis on the basis of Systemic Inflammatory Response Syndrome to be confirmed by blood culture were enrolled in the study after informed consent. Patients with renal dysfunction and congenital anomalies were excluded. Blood samples were taken for blood culture and Presepsin levels. Presepsin levels were measured by enzyme linked immunosorbent assay (ELISA). Data were analysed by SPSS-20. Quantitative variables were presented as median (IQR) or mean ± SD depending on data distribution. Qualitative variables were presented as frequency and percentage. ROC curve was plotted to determine the optimal cut-off value of Presepsin levels to differentiate between sepsis and non-sepsis. Sensitivity (Sn), specificity (Sp), positive predictive value (PPV), and negative predictive value (NPV) of Presepsin were calculated.ResultsThe median (IQR) age and total leukocyte count, and male: female ratio was not significantly different in patients with and without sepsis. Using 600 pg/ml cut-off value, Sn, Sp, PPV, and NPV for Presepsin were calculated as 72.73, 71.43, 62 and 81%, respectively.ConclusionSerum Presepsin level has overall good diagnostic accuracy to diagnose bacterial sepsis.Key WordsPresepsin, Systemic Inflammatory Response Syndrome, Bacterial sepsis, Paediatric patients.

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