• Luz M Garduño-Zarazúa, Dora G Mayén, Ricardo Meléndez-Hernández, Antonio J Paz-Martínez, Eva Ramírez-Arroyo, David Sosa, Mónica Aguinaga, Javier Pérez-Durán, Osiris Ríos, Jaime López-Valdez, Leda Torres, and Sara Frias.
• Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico.
• Gac Med Mex. 2024 Jan 1; 160 (1): 768576-85.

BackgroundChromosomal abnormalities are present in 50 to 60% of miscarriages and in 6 to 19% of stillbirths. Although microarrays are preferred for studying chromosomal abnormalities, many hospitals cannot offer this methodology.ObjectiveTo present the results of the cytogenetic analysis of 303 products of conception (POC), which included 184 miscarriages, 49 stillbirths and 17 cases of undefined age.Material And MethodsKaryotyping, fluorescence in situ hybridization, short tandem repeats and microarrays were used, depending on the type of loss and available sample.ResultsIn 29 POCs we found maternal tissue and were eliminated from the analyses. Informative results were obtained in 250 (91.2 %)/274 cases; the karyotyping success rate was 80.7%; that of single nucleotide polymorphism microarrays, 94.5%; and that of fluorescence in situ hybridization and short tandem repeat, 100%. Cytogenetic abnormalities were observed in 57.6% of miscarriages and in 24.5% of stillbirths; 94% of total anomalies were numerical and 6% were submicroscopic.ConclusionsKaryotyping with simultaneous short tandem repeat study to rule out contamination of maternal cells is effective for studying miscarriages; in stillbirths, microarrays are recommended.Copyright: © 2024 Permanyer.

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