• Mustafa C Durmuşlar, Elif A Gülşen, Kemal A Bayburt, Umut Ballı, Mert Ocak, Ayşegül Fırat, Elham B Zırh, and Hakan H Çelik.
• From the Department of Oral and Maxillofacial Surgery (Durmuşlar, Bayburt), Faculty of Dentistry Biruni University, Istanbul, from the Department of Oral and Maxillofacial Surgery (Gülşen), Ibn-i Sina Campus Faculty of Dentistry Bulent Ecevit University Esenkoy, Zonguldak, from the Department of Periodontology (Ballı), Adana Yüreğir Karşıyaka Oral and Dental Health Hospital, Adana, from the Department of Basic Medical Sciences, Anatomy (Ocak), Faculty of Dentistry, Ankara University, from the Department of Anatomy (Fırat, Çelik), Faculty of Medicine, Hacettepe University, and from the Department of Histology and Embryology (Zırh), Faculty of Medicine, TOBB University of Economics and Tecnology, Ankara, Turkey.
• Saudi Med J. 2024 Aug 1; 45 (8): 791798791-798.

ObjectivesTo assess the impact of autogenous graft materials, including autogenous bone graft (ABG) and injectable platelet-rich fibrin (I-PRF) clots, on bone defect regeneration in ovariectomized osteoporotic rats.MethodsWistar rats (6-8 weeks old) were ovariectomized, and surgery began after 8 weeks. A 5-mm defect was created bilaterally in the parietal bones of 16 rats, which were divided into 4 groups. Group A (blank) had untreated defects; group B had defects filled with 0.1 ml of I-PRF, group C had defects filled with 0.1 ml of ABG, and group D had defects filled with a combination of 0.1 ml each of I-PRF and ABG. Four weeks post-surgery, the rats were euthanized. Bone regeneration was evaluated through histopathologic analysis and microcomputed tomography (micro-CT).ResultsIn ovariectomized rats treated with ABG or I-PRF, bone regeneration was enhanced, with increased periosteal activity, osteoblast count, and new bone volume, as determined histologically. The ABG+I-PRF group had the highest periosteal vascularity, but the difference compared to the ABG group was not statistically significant (p>0.05). Osteoblast numbers were significantly higher in the ABG+I-PRF group than in the blank group (p<0.05). Micro-CT showed the highest mean new bone volume ratio in the ABG+I-PRF group, followed by the ABG group.ConclusionThe combined use of ABG and I-PRF enhances bone formation in osteoporotic rats following ovariectomy.Copyright: © Saudi Medical Journal.

19 keywords...

hide…