• Meiyi Chen, Shaomeng Sun, Lei Ma, Li Bian, Biao Zhao, and Haiyong Zeng.
• Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, Shanghai, 200065, China.
• Ir J Med Sci. 2025 Feb 13.

BackgroundThe pivotal role of microRNAs (miRNAs) in the pathogenesis of gestational diabetes mellitus (GDM) have been increasingly highlighted in recent studies.AimsTo investigate miR-144-3p expression in patients with GDM and its potential mechanisms in GDM.MethodsAccording to the inclusion criteria, 98 pregnant women with GDM and 98 normal pregnant women were statistically selected. The levels of miR-144-3p were determined by RT-qPCR. The ROC curve was used to evaluate the diagnostic value of miR-144-3p for GDM. Pearson analysis was used to analyze the correlation. HRT-8/SVneo cells were induced by high glucose in vitro to mimic the high glucose environment of GDM. On this basis, the cell models of miR-144-3p overexpression and knockdown were established to investigate the potential mechanism of miR-144-3p in GDM.ResultsThe level of miR-144-3p in GDM group was markedly higher than that in normal pregnant women. In terms of predicting GDM, miR-144-3p demonstrated high accuracy. MiR-144-3p was positively linked to blood glucose markers in GDM. Further investigation has confirmed that miR-144-3p harbors a binding site for FOXO1 and can directly modulate its expression. Moreover, miR-144-3p targeted FOXO1 to affect the proliferation of trophoblast cells.ConclusionThe expression of miR-144-3p is elevated in pregnant women with GDM. MiR-144-3p may be involved in the regulation of GDM by targeting FOXO1.© 2025. The Author(s), under exclusive licence to Royal Academy of Medicine in Ireland.

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