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Brain research bulletin · Mar 2001
Spider neurotoxins block the beta scorpion toxin-induced calcium uptake in rat brain cortical synaptosomes.
- D M Miranda, M A Romano-Silva, E Kalapothakis, C R Diniz, M N Cordeiro, T Moraes-Santos, L De Marco, M A Prado, and M V Gomez.
- Laboratírio de Neurofarmacologia, Departamento de Farmacologia, ICB-UFMG, MG, Belo Horizonte, Brazil.
- Brain Res. Bull. 2001 Mar 15;54(5):533-6.
AbstractIn this paper we describe the effects of the beta scorpion toxin Tityus gamma (TiTX gamma) and spider neurotoxins Tx3-3 and Tx3-4 in the (45)Ca(2+) uptake in synaptosomes. The TiTX gamma-stimulatory effect on (45)Ca(2+) uptake in synaptosomes was inhibited omega-Conotoxin MVIIC (omega-CgTX MVIIC) (0.1 microM) and omega-Agatoxin IVA (0.1 microM) by 70% and 41%, respectively. omega-CgTX MVIIC (1.0 microM) almost completely blocked the TiTX gamma-induced (45)Ca(2+) uptake in synaptosomes. Verapamil (1.0 microM) and omega-Conotoxin GVIA (0.1 microM) had no effect in the scorpion toxin-induced (45)Ca(2+) influx. The spider neurotoxins Tx3-3 and Tx3-4 inhibited the TiTX gamma-induced calcium uptake with an IC(50) of 10.0 and 30.0 nM, respectively. It is suggested that spider neurotoxins Tx3-3 and Tx3-4 blocking effect in the TiTX gamma-induced calcium uptake involves P/Q-type calcium channels.
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