• Ilse Vanhorebeek, Jan Gunst, Sarah Derde, Inge Derese, Magaly Boussemaere, Fabian Güiza, Wim Martinet, Jean-Pierre Timmermans, André D'Hoore, Pieter J Wouters, and Greet Van den Berghe.
• Department of Intensive Care Medicine, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium. ilse.vanhorebeek@med.kuleuven.be
• J. Clin. Endocrinol. Metab. 2011 Apr 1;96(4):E633-45.

ContextResponses to critical illness, such as excessive inflammation and hyperglycemia, may trigger detrimental chain reactions that damage cellular proteins and organelles. Such responses to illness contribute to the risk of (nonresolving) multiple organ dysfunction and adverse outcome.ObjectiveWe studied autophagy as a bulk degradation pathway able to remove toxic protein aggregates and damaged organelles and how these are affected by preventing hyperglycemia with insulin during critical illness.Design And SettingPatients participated in a randomized study, conducted at a university hospital surgical/medical intensive care unit.PatientsWe studied adult prolonged critically ill patients vs. controls.InterventionsTolerating excessive hyperglycemia was compared with intensive insulin therapy targeting normoglycemia.Main Outcome MeasuresWe quantified (ultra)structural abnormalities and hepatic and skeletal muscle protein levels of key players in autophagy.ResultsMorphologically, both liver and muscle revealed an autophagy-deficiency phenotype. Proteins involved in initiation and elongation steps of autophagy were induced 1.3- to 6.5-fold by critical illness (P ≤ 0.01), but mature autophagic vacuole formation was 62% impaired (P = 0.05) and proteins normally degraded by autophagy accumulated up to 97-fold (P ≤ 0.03). Mitophagy markers were unaltered or down-regulated (P = 0.05). Although insulin preserved hepatocytic mitochondrial integrity (P = 0.05), it further reduced the number of autophagic vacuoles by 80% (P = 0.05).ConclusionsInsufficient autophagy in prolonged critical illness may cause inadequate removal of damaged proteins and mitochondria. Such incomplete clearance of cellular damage, inflicted by illness and aggravated by hyperglycemia, could explain lack of recovery from organ failure in prolonged critically ill patients. These data open perspectives for therapies that activate autophagy during critical illness.

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