• Guixiang Chen, Yang Zhang, Xiaohong Jin, Lihong Zhang, Yunjiao Zhou, Jianying Niu, Jing Chen, and Yong Gu.
• Division of Nephrology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, P. R. China.
• Proteomics Clin Appl. 2011 Jun 1;5(5-6):300-10.

PurposeAs a noninvasive examination, urinary proteomics is a very useful tool to identify renal disease. The purpose of the present study was to find differential proteins among women with preeclampsia, gestational hypertension and normal pregnancy, and to screen potential biomarkers for the early diagnosis of preeclampsia.Experimental DesignUrinary proteins were identified by iTRAQ labeling coupled with 2-D LC-MS/MS. The bioinformatics analysis was performed with the Metacore software and the International Protein Index (IPI) and the Gene Ontology (GO) Database. The differentially expressed proteins were verified by ELISA.Results362 nonredundant proteins were identified, 113 of which were expressed differentially between preeclampsia and normal pregnant group and 31 differential proteins among three groups. These differential proteins were associated with biological processes of blood coagulation, cell adhesion and differentiation, immune response and cytoskeleton development, etc. They interacted with each other in the network. The urinary angiotensinogen (AGT) was downregulated, which was consistent with the ELISA validation results.Conclusions And Clinical RelevanceThe present study found a multitude of differential proteins that might provide a clue for investigating the mechanism of proteinuria development in preeclampsia. Low urinary angiotensinogen levels were useful for identifying preeclampsia.Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

29 keywords...

hide…