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- Aurélie Facomprez, Emmanuel Beaurepaire, and Delphine Débarre.
- Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, 91128 Palaiseau, France.
- Opt Express. 2012 Jan 30;20(3):2598-612.
AbstractWe investigate theoretically and experimentally the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. On the example of two-photon fluorescence imaging, we show that using a suitable number of measurements, precise correction can be obtained for up to 2 radians rms aberrations without optimising the aberration modes used for correction. We also investigate the number of photons required for accurate correction when signal acquisition is shot-noise limited. We show that only 10(4) to 10(5) photons are required for complete correction so that the correction process can be implemented with limited extra-illumination and associated photoperturbation. Finally, we provide guidelines for implementing an optimal correction algorithm depending on the experimental conditions.
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