• Methods Mol. Biol. · Jan 2010

    Delivery of RNA interference to peripheral neurons in vivo using herpes simplex virus.

    • Anna-Maria Anesti.
    • BioVex Inc, Woburn, MA, USA. MAnesti@biovex.com
    • Methods Mol. Biol. 2010 Jan 1;617:347-61.

    AbstractRNA interference (RNAi) has become a powerful tool for modulating gene expression. While delivery of small interfering RNAs (siRNAs) has achieved silencing of pain-related genes in various animal models of nociception, delivery of short-hairpin RNA (shRNA) or artificial miRNA (miRNA) to dorsal root ganglia (DRG) has proven particularly challenging. This chapter describes a highly efficient method for in vivo gene silencing in sensory neurons using replication-defective vectors based on herpes simplex virus (HSV). This method can be utilised to obtain a better understanding of gene function, validate novel gene targets in drug discovery and potentially develop new RNAi-mediated approaches to achieve analgesia.

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