• Christine M Oshansky, Andrew J Gartland, Sook-San Wong, Trushar Jeevan, David Wang, Philippa L Roddam, Miguela A Caniza, Tomer Hertz, John P Devincenzo, Richard J Webby, and Paul G Thomas.
• 1 Department of Immunology, and.
• Am. J. Respir. Crit. Care Med.. 2014 Feb 15;189(4):449-62.

RationaleChildren are an at-risk population for developing complications following influenza infection, but immunologic correlates of disease severity are not understood. We hypothesized that innate cellular immune responses at the site of infection would correlate with disease outcome.ObjectivesTo test the immunologic basis of severe illness during natural influenza virus infection of children and adults at the site of infection.MethodsAn observational cohort study with longitudinal sampling of peripheral and mucosal sites in 84 naturally influenza-infected individuals, including infants. Cellular responses, viral loads, and cytokines were quantified from nasal lavages and blood, and correlated to clinical severity.Measurements And Main ResultsWe show for the first time that although viral loads in children and adults were similar, innate responses in the airways were stronger in children and varied considerably between plasma and site of infection. Adjusting for age and viral load, an innate immune profile characterized by increased nasal lavage monocyte chemotactic protein-3, IFN-α2, and plasma IL-10 levels at enrollment predicted progression to severe disease. Increased plasma IL-10, monocyte chemotactic protein-3, and IL-6 levels predicted hospitalization. This inflammatory cytokine production correlated significantly with monocyte localization from the blood to the site of infection, with conventional monocytes positively correlating with inflammation. Increased frequencies of CD14(lo) monocytes were in the airways of participants with lower inflammatory cytokine levels.ConclusionsAn innate profile was identified that correlated with disease progression independent of viral dynamics and age. The airways and blood displayed dramatically different immune profiles emphasizing the importance of cellular migration and localized immune phenotypes.

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