• European radiology · Sep 2014

    T1-mapping for assessment of ischemia-induced acute kidney injury and prediction of chronic kidney disease in mice.

    • Katja Hueper, Matti Peperhove, Song Rong, Jessica Gerstenberg, Michael Mengel, Martin Meier, Marcel Gutberlet, Susanne Tewes, Amelie Barrmeyer, Rongjun Chen, Herman Haller, Frank Wacker, Dagmar Hartung, and Faikah Gueler.
    • Department of Radiology, Hannover Medical School, Carl-Neuberg-Str.1, 30625, Hannover, Germany, hueper.katja@mh-hannover.de.
    • Eur Radiol. 2014 Sep 1;24(9):2252-60.

    ObjectivesTo investigate whether T1-mapping allows assessment of acute kidney injury (AKI) and prediction of chronic kidney disease (CKD) in mice.MethodsAKI was induced in C57Bl/6N mice by clamping of the right renal pedicle for 35 min (moderate AKI, n = 26) or 45 min (severe AKI, n = 23). Sham animals served as controls (n = 9). Renal histology was assessed in the acute (day 1 + day 7; d1 + d7) and chronic phase (d28) after AKI. Furthermore, longitudinal MRI-examinations (prior to until d28 after surgery) were performed using a 7-Tesla magnet. T1-maps were calculated from a fat-saturated echoplanar inversion recovery sequence, and mean and relative T1-relaxation times were determined.ResultsRenal histology showed severe tubular injury at d1 + d7 in both AKI groups, whereas, at d28, only animals with prolonged 45-min ischemia showed persistent signs of AKI. Following both AKI severities T1-values significantly increased and peaked at d7. T1-times in the contralateral kidney without AKI remained stable. At d7 relative T1-values in the outer stripe of the outer medulla were significantly higher after severe than after moderate AKI (138 ± 2% vs. 121 ± 3%, p = 0.001). T1-elevation persisted until d28 only after severe AKI. Already at d7 T1 in the outer stripe of the outer medulla correlated with kidney volume loss indicating CKD (r = 0.83).ConclusionT1-mapping non-invasively detects AKI severity in mice and predicts further outcome.Key PointsRenal T1-relaxation times are increased after ischemia-induced acute kidney injury. Renal T1-values correlate with subsequent kidney volume loss. T1-mapping detects the severity of acute kidney injury and predicts further outcome.

      Pubmed     Full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…