• Matthew S Halquist and H Thomas Karnes.
• Virginia Commonwealth University, Department of Pharmaceutics, PO Box 980533, Richmond, Virginia 980533, USA.
• Biomed. Chromatogr. 2011 Jan 1; 25 (1-2): 47-58.

AbstractTargeted protein quantification using peptide surrogates has increasingly become important to the validation of biomarker candidates and development of protein therapeutics. These approaches have been proposed and employed as alternatives to immunoassays in biological fluids. Technological advances over the last 20 years in biochemistry and mass spectrometry have prompted the use of peptides as surrogates to quantify enzyme digested proteins using triple quadrupole mass spectrometers. Multiple sample preparation processes are often incorporated to achieve quantification of target proteins using these signature peptides. This review article focuses on these processes or hyphenated techniques for quantification of proteins with peptide surrogates. The most recent advances and strategies involved with hyphenated techniques are discussed.2010 John Wiley & Sons, Ltd.

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