Biomedical chromatography : BMC
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Biomed. Chromatogr. · Jan 2011
ReviewQuantitative liquid chromatography tandem mass spectrometry analysis of macromolecules using signature peptides in biological fluids.
Targeted protein quantification using peptide surrogates has increasingly become important to the validation of biomarker candidates and development of protein therapeutics. These approaches have been proposed and employed as alternatives to immunoassays in biological fluids. ⋯ This review article focuses on these processes or hyphenated techniques for quantification of proteins with peptide surrogates. The most recent advances and strategies involved with hyphenated techniques are discussed.