-
- Mônica Lisboa Chang Wayhs, Mauro Batista de Morais, Ubiratan Fabres Machado, Silvia Modesto Nassar, Ulysses Fagundes Neto, and Olga Maria Silvério Amâncio.
- Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil.
- Nutrition. 2011 Jan 1; 27 (1): 111-115.
ObjectiveTo evaluate the transepithelial transport of sodium, glucose, potassium, and water and the mRNA level of the sodium-glucose cotransporter (SGLT1) and the facilitated sugar transporter (GLUT2) in the small intestine of iron-deficient rats.MethodsAfter 6 wk of receiving diets with low or normal iron content, rats (Wistar-EPM) were subjected to two experiments: 1) evaluation of the transepithelial transport of sodium, glucose, potassium, and water by an "in vivo" experimental model of intestinal perfusion and 2) determination of relative SGLT1 and GLUT2 mRNA levels in the proximal, intermediate, and distal portions of the small intestine by the northern blotting technique.ResultsHemoglobin and hepatic iron levels were statistically lower in the anemic rats. The mean transepithelial transports of sodium (-33.0 μEq · min(-1) · cm(-1), glucose (426.0 μM · min(-1) · cm(-1), and water (0.4 μL · min(-1) · cm(-1) in the small intestine of the anemic rats were significantly lower than in the control group (349.1 μEq · min(-1) · cm(-1), 842.6 μM · min(-1) · cm(-1), and 4.3 μl · min(-1) · cm(-1), respectively, P < 0.05). The transepithelial transport of potassium was similar for both groups. The relative SGLT1 mRNA levels of the anemic rats in the intermediate (1.796 ± 0.659 AU) and distal (1.901 ± 0.766 AU) segments were significantly higher than the values for the control rats (intermediate 1.262 ± 0.450 AU, distal 1.244 ± 0.407 AU). No significant difference was observed for the relative SLGT1 mRNA levels in the proximal segment or for the GLUT2 mRNA levels in all segments.ConclusionIron deficiency decreases the absorption of glucose, sodium, and water and increases SGLT1 mRNA in the intermediate and distal segments of the small intestine of rats.Copyright © 2011 Elsevier Inc. All rights reserved.
Notes
Knowledge, pearl, summary or comment to share?You can also include formatting, links, images and footnotes in your notes
- Simple formatting can be added to notes, such as
*italics*,_underline_or**bold**. - Superscript can be denoted by
<sup>text</sup>and subscript<sub>text</sub>. - Numbered or bulleted lists can be created using either numbered lines
1. 2. 3., hyphens-or asterisks*. - Links can be included with:
[my link to pubmed](http://pubmed.com) - Images can be included with:
 - For footnotes use
[^1](This is a footnote.)inline. - Or use an inline reference
[^1]to refer to a longer footnote elseweher in the document[^1]: This is a long footnote..