• X Liu, B Zhang, H Yang, H Wang, Y Liu, A Huang, T Liu, X Tian, Y Tong, T Zhou, T Zhang, G Xing, W Xiao, X Guo, D Fan, X Han, G Liu, Z Zhou, and D Chui.
• Neuroscience Research Institute & Department of Neurobiology, Key Laboratory for Neuroscience of Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing 100191, China.
• Neuroscience. 2014 Nov 7; 280: 275-81.

AbstractLipoprotein lipase (LPL) is expressed at high levels in hippocampal neurons, although its function is unclear. We previously reported that LPL-deficient mice have learning and memory impairment and fewer synaptic vesicles in hippocampal neurons, but properties of synaptic activity in LPL-deficient neurons remain unexplored. In this study, we found reduced frequency of miniature excitatory postsynaptic currents (mEPSCs) and readily releasable pool (RRP) size in LPL-deficient neurons, which led to presynaptic dysfunction and plasticity impairment without altering postsynaptic activity. We demonstrated that synaptic vesicle recycling, which is known to play an important role in maintaining the RRP size in active synapses, is impaired in LPL-deficient neurons. Moreover, lipid assay revealed deficient docosahexaenoic acid (DHA) and arachidonic acid (AA) in the hippocampus of LPL-deficient mice; exogenous DHA or AA supplement partially restored synaptic vesicle recycling capability. These results suggest that impaired synaptic vesicle recycling results from deficient DHA and AA and contributes to the presynaptic dysfunction and plasticity impairment in LPL-deficient neurons. Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

36 keywords...

hide…