-
J. Clin. Microbiol. · Apr 2014
Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry.
- Yu-Tzu Chang, Hsuan-Chen Wang, Ming-Cheng Wang, An-Bang Wu, Junne-Ming Sung, H Sunny Sun, Ih-Jen Su, Wei-Chih Kan, Chih-Chiang Chien, Jyh-Chang Hwang, Hsien-Yi Wang, Chin-Chung Tseng, and Chi-Jung Wu.
- Department of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan.
- J. Clin. Microbiol. 2014 Apr 1; 52 (4): 1217-9.
AbstractPCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.
Notes
Knowledge, pearl, summary or comment to share?You can also include formatting, links, images and footnotes in your notes
- Simple formatting can be added to notes, such as
*italics*,_underline_or**bold**. - Superscript can be denoted by
<sup>text</sup>and subscript<sub>text</sub>. - Numbered or bulleted lists can be created using either numbered lines
1. 2. 3., hyphens-or asterisks*. - Links can be included with:
[my link to pubmed](http://pubmed.com) - Images can be included with:
 - For footnotes use
[^1](This is a footnote.)inline. - Or use an inline reference
[^1]to refer to a longer footnote elseweher in the document[^1]: This is a long footnote..