• J. Clin. Microbiol. · Apr 2014

    Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry.

    • Yu-Tzu Chang, Hsuan-Chen Wang, Ming-Cheng Wang, An-Bang Wu, Junne-Ming Sung, H Sunny Sun, Ih-Jen Su, Wei-Chih Kan, Chih-Chiang Chien, Jyh-Chang Hwang, Hsien-Yi Wang, Chin-Chung Tseng, and Chi-Jung Wu.
    • Department of Internal Medicine, National Cheng Kung University Hospital and College of Medicine, Tainan, Taiwan.
    • J. Clin. Microbiol. 2014 Apr 1; 52 (4): 1217-9.

    AbstractPCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

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