• Colm M O'Grada, Melissa J Morine, Ciara Morris, Miriam Ryan, Eugene T Dillon, Marianne Walsh, Eileen R Gibney, Lorraine Brennan, Michael J Gibney, and Helen M Roche.
• Nutrigenomics Research Group, UCD Conway Institute of Biomolecular and Biomedical Research, School of Public Health and Population Science, University College Dublin, Belfield, Dublin, Ireland; Institute o... more f Food and Health, University College Dublin, Belfield, Dublin, Ireland. less
• Mol Nutr Food Res. 2014 Apr 1; 58 (4): 808-20.

ScopeFood and nutrition studies often require accessing metabolically active tissues, including adipose tissue. This can involve invasive biopsy procedures that can be a limiting factor in study design. In contrast, peripheral blood mononuclear cells (PBMCs) are a population of circulating immune cells that are easily accessible through venipuncture. As transcriptomics is of growing importance in food and metabolism research, understanding the transcriptomic relationship between these tissue types can provide insight into the utility of PBMCs in this field.Methods And ResultsWe examine this relationship within eight subjects, in two postprandial states (following oral lipid tolerance test and oral glucose tolerance test). Multivariate analysis techniques were used to examine variation between tissues, samples, and subjects in order to define which genes havecommon/disparate expression profiles associated with highly defined metabolic phenotypes. We demonstrate global similarities in gene expression between PBMCs and white adipose tissue, irrespective of the metabolic challenge type. Closer examination of individual genes revealed this similarity to be strongest in pathways related to immune response/inflammation. Notably, the expression of metabolism-related nuclear receptors, including PPARs, LXR, etc. was discordant between tissuesConclusionThe PBMC transcriptome may therefore provide a unique insight into the inflammatory component of metabolic health, as opposed to directly reflecting the metabolic component of the adipose tissue transcriptome.© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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