Neuroscience
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A monoclonal antibody, 2G12 , has been produced against a rat cerebral cortex glycoprotein fraction. It interacts with Thy-1 based on both the tissue distribution of its reactivity and the blocking of its binding by pretreatment with a rabbit anti-Thy-1 serum. In rat retina this antibody labels only cell bodies in the ganglion cell layer, optic nerve fibres and the inner plexiform layer. ⋯ There was almost complete coincidence of the two labels. A monoclonal antibody against mouse Thy-1.2 gave an essentially identical pattern of labelling. In both rats and mice Thy-1 was also found on the vitreal surface of the inner limiting membrane in a pattern reminiscent of that formed by the Müller cell endfeet, although these cells do not express Thy-1.