Brain research bulletin
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Brain research bulletin · Jul 2000
Ketamine blockage of both tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels of rat dorsal root ganglion neurons.
Ketamine, a general anesthetic, has been reported to block sodium channels. Two types of Na(+) channels, tetrodotoxin (TTX)-sensitive (TTX-s) and TTX-resistant (TTX-r), are expressed in dorsal root ganglion (DRG) neurons. The present study was to investigate the effects of ketamine on both types, particularly on TTX-r channels, using whole-cell patch-clamp recordings in dissociated rat DRG neurons. ⋯ Activation and inactivation properties of both TTX-s and TTX-r Na(+) channels were affected by ketamine. Since TTX-r Na(+) channels were preferentially expressed in small DRG neurons known as nociceptors, blockage of TTX-r Na(+) channels by ketamine may result in reducing nociceptive signals conducting to the spinal cord. Moreover, both TTX-r and TTX-s Na(+) channels would be non-selectively blocked by ketamine at high concentration, suggesting that the high dose of ketamine might produce an action of local anesthesia.
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Brain research bulletin · Jul 2000
Vestibular-induced behaviour of rats born and raised in hypergravity.
One group of rats were bred and kept under hypergravity (HG) conditions (2.5 g) in a centrifuge. Another group were bred and kept under normal gravity conditions (1 g). Rats from both groups were dropped from a supine position into a water basin under infrared illumination leaving only gravity (1 g for both groups) for orientation. ⋯ Surfacing of young HG rats is less successful (36% at age 6 weeks) and requires more time. On average, surfacing of adult rats of both groups is about the same. Apparently, the repeated stay of centrifuge-bred rats at 1 g for experiments and daily care suffices to recalibrate and improve their orientation, which is essential for surfacing.