The Tokai journal of experimental and clinical medicine
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Tokai J. Exp. Clin. Med. · Jan 2013
A stable in vitro method for assessing the toxicity of potassium cyanide and its antidote.
Hydrogen cyanide possesses a high acid-dissociation constant of 9.14, favoring its vaporization and depletion from the culture media at physiological pH, which may cause the cyanide toxicity unstable in vitro. ⋯ Adjustment of culture medium pH to 9.2 could stabilize cyanide concentration and decrease the effective concentration of KCN, allowing stable evaluation of KCN toxicity and antidotal efficacy.