Journal of cancer research and clinical oncology
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J. Cancer Res. Clin. Oncol. · Jan 1995
Inhibitory effect of cold stress on lung tumours induced by 7,12-dimethylbenz[a]anthracene in mice.
The effect of cold stress on lung tumours induced by 7,12-dimethylbenz[a]anthracene (DMBA) was investigated in ICR male and female mice. When mice were exposed to cold stress at 0 +/- 1 degree C for 2 h, three times per week (every other day) for 3 months, the rectal temperatures and hepatic glutathione levels were significantly decreased. ⋯ In addition, although DMBA (1 mg/kg) caused lung tumours in 20% or 40% of the treated male or female mice 4 months after injection, it did not cause lung tumours in all of the male and female mice exposed to cold stress. These results suggest that cold stress may inhibit lung tumours induced by chemicals.
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J. Cancer Res. Clin. Oncol. · Jan 1995
Nerve-growth-factor-dependent neurite outgrowth assay; a research model for chemotherapy-induced neuropathy.
The rat clonal pheochromocytoma cell line PC12 can be induced to display neurite outgrowth upon induction with nerve growth factor (NGF). This NGF-dependent neurite outgrowth assay looks a promising model for research on toxic neuropathies. Using this assay we demonstrated that cisplatin caused a dose-dependent reduction of NGF-dependent neurite formation. ⋯ Even at a high cisplatin concentration (10 micrograms/ml), the protective action of NGF, although less adequate, was observed. The value and strength of this model for screening neuropathogenic effects of anticancer agents at the cellular level and the possibly therapeutic action of neurotrophins are discussed and demonstrated. Furthermore, in the light of the urgent need for adequate models for neuropathy research, the PC12 neurite outgrowth protection assay may contribute to our knowledge of the mechanisms underlying the development of neuropathy.