Archivos de bronconeumología
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Arch. Bronconeumol. · Jun 2014
Clinical TrialViability of lymph node samples obtained by echobronchoscopy in the study of epigenetic alterations in patients with lung cancer.
The diagnosis of microscopic lymph node metastasis in lung cancer is challenging despite the constant advances in tumor staging. The analysis of the methylation status of certain genes in lymph node samples could improve the diagnostic capability of conventional cyto-histological methods. The aim of this study was to demonstrate the feasibility of methylation studies using cytological lymph node samples. ⋯ Methylation quantification using MS-qPCR and pyrosequencing of cytological lymph node samples obtained using echobronchoscopy is feasible if an appropriate DNA concentration is obtained, notably contributing to the identification of epigenetic biomarkers capable of improving decision-making for the benefit of potentially curable lung cancer patients.
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Arch. Bronconeumol. · Jun 2014
Real-time prediction of mediastinal lymph node malignancy by endobronchial ultrasound.
To evaluate the utility of different ultrasonographic (US) features in differentiating benign and malignant lymph node (LN) by endobronchial ultrasound (EBUS) and validate a score for real-time clinical application. ⋯ Combination of different US criteria can be useful for prediction of mediastinal LN malignancy and valid for real-time clinical application.