Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
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J Coll Physicians Surg Pak · Oct 2021
Detection of Kras Gene in Colorectal Cancer Patients through Liquid Biopsy: A Cost-effective Method.
To detect the Kras gene through liquid biopsy, a less invasive technique in diagnosed colorectal cancer patients. ⋯ Kras gene was detected with significantly increased levels in plasma of CRC patients at advanced stages. This confirms that liquid biopsy can be used to detect Kras gene in ctDNA of CRC patients through a magnetic bead based technique. Key Words: Liquid biopsy, Circulating tumor DNA, KRAS, Colorectal cancer, Real-time polymerase chain reaction.
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J Coll Physicians Surg Pak · Oct 2021
Observational StudyCorrelation between Intestinal Flora and Serum Inflammatory Factors in Post-stroke Depression in Ischemic Stroke.
To investigate the correlation between intestinal flora and serum inflammatory factors IL-1, IL-2, IL-6 and hs-CRP in post-stroke depression (PSD) in ischemic stroke patients. ⋯ There are intestinal flora imbalance and Bifidobacterium undergrowth in patients with PSD, which can lead to overexpression of serum inflammatory factors. Both may be involved in occurrence and progress of PSD in patients with ischemic stroke. Key Words: Ischemic stroke, Post-stroke depression (PSD), Intestinal flora, Inflammatory factors.
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J Coll Physicians Surg Pak · Oct 2021
Relative Risk of Anxiety and Depression among COVID-19 Survivor Healthcare Workers from a Tertiary Care Hospital in Pakistan: A Pilot Cohort Study.
Longitudinal studies for determining the risk of anxiety and depression among COVID-19 survivor healthcare workers are lacking. This study aimed to determine the risk of anxiety and depression among healthcare workers that survived COVID-19 infection through a six-month post-recovery follow-up. This was a cohort study conducted at the Department of Obstetrics and Gynecology, Lady Reading Hospital, Peshawar, from 1st March to 30th August 2020. ⋯ There was no increased risk of anxiety or depression six months post-COVID-19 infection in the infected HCW, compared to uninfected HCW. It is recommended to conduct longitudinal studies with large sample and longer follow-up. Key Words: Relative risk, Anxiety, Depression, HADS, COVID-19, Healthcare workers.
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J Coll Physicians Surg Pak · Oct 2021
Case ReportsA Special Case of Cirrhosis with a Novel ATP7B Mutation and Occult Chronic HBV Infection.
Wilson's disease (WD) is an autosomal recessive disorder that affects copper metabolism. Mutations of the ATP7B gene have been found to be strongly associated with a risk of developing WD; and at present, more than 500 mutations have been reported in WD patients. The Arg778Leu and Arg952Lys mutations in exons 8 and 12, respectively, are highly prevalent in the Chinese population. ⋯ Meanwhile, the early stage of occult hepatitis B infection lacks typical clinical manifestations, which easily leads to it being misdiagnosed as liver cirrhosis. We report a new pathogenic exon 19 mutation of ATP7B，which can potentially contribute to the early genetic diagnosis and prompt treatment of WD. Key Words: Wilson's disease, Gene mutation, ATP7B, Cirrhosis, Occult hepatitis B infection.
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J Coll Physicians Surg Pak · Oct 2021
Observational StudyLocating Adductor Canal and Quantifying the Median Effective Volume of Ropivacaine for Adductor Canal Block by Ultrasound.
To identify the precise position of adductor canal; and to determine median effective volume (EV50) for adductor canal block (ACB) by ultrasound. ⋯ The midpoint between the femoral trochanter and the superior margin of patella is in the adductor canal. EV50 of 0.5% ropivacaine for ACB is 10.79 ml (95% CI, 10.10-11.52 ml). Key Words: Adductor canal, Location, Ultrasound, Median effective volume, Nerve block.