Journal of neurophysiology
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Using an in vitro nerve skin preparation and controlled mechanical or thermal stimuli, we analyzed the receptive properties of 277 mechanosensitive single primary afferents with myelinated (n = 251) or unmyelinated (n = 26) axons innervating the hairy skin in adult or 2-wk-old mice. Afferents were recorded from small filaments of either sural or saphenous nerves in an outbred mice strain or in the inbred Balb/c strain. On the basis of their receptive properties and conduction velocity, several receptor types could be distinguished. ⋯ In juvenile mice, 22% of RA units also displayed an SA response at high stimulus intensities; these units were termed RA/SA units. We conclude that all types of cutaneous afferent fibers are already committed to their phenotype 2 wk after birth but undergo some maturation over the following weeks. This preparation has great potential for the study of transgenic mice with targeted mutations of genes that code factors that are involved in the specification of sensory neuron phenotypes.
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Impulses in afferent C fibers, e.g., during peripheral trauma, may induce plastic changes in the spinal dorsal horn that are believed to contribute to some forms of hyperalgesia. The nature of lasting changes in spinal nociception are still not well understood. Here we characterized the long-term potentiation (LTP) of spinal field potentials with a negative focus in superficial spinal dorsal horn evoked by supramaximal electrical stimulation of the sciatic nerve in urethan-anesthetized adult rats. ⋯ Thus the activation of neurokinin receptors is necessary for the induction but not for the maintenance of LTP of C-fiber-evoked field potentials in spinal dorsal horn. This model may be useful to study plastic changes in spinal cord induced by peripheral C-fiber stimulation. The LTP of C-fiber-evoked field potentials may be a mechanism underlying some forms of hyperalgesia.
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Clinical Trial
Tangential torque effects on the control of grip forces when holding objects with a precision grip.
When we manipulate small objects, our fingertips are generally subjected to tangential torques about the axis normal to the grasp surface in addition to linear forces tangential to the grasp surface. Tangential torques can arise because the normal force is distributed across the contact area rather than focused at a point. We investigated the effects of tangential torques and tangential forces on the minimum normal forces required to prevent slips (slip force) and on the normal forces actually employed by subjects to hold an object in a stationary position with the use of the tips of the index finger and thumb. ⋯ There was no difference in safety margin between the digits. In conclusion, tangential torque strongly influences the normal force required for grasp stability. When controlling normal force, people take into account, in a precise fashion, the slip force reflecting both tangential force and tangential torque and their interaction as well as the current frictional condition in the object-digit interface.
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Previous studies have shown that when monkeys perform a delayed match-to-sample (DMS) task, some neurons in inferotemporal visual cortex are activated selectively during the delay period when the animal must remember particular visual stimuli. This selective delay activity may be involved in short-term memory. It does not depend on visual stimulation: both auditory and tactile stimuli can trigger selective delay activity in inferotemporal cortex when animals expect to respond to visual stimuli in a DMS task. ⋯ In the first animal, a specific long-term memory representation for learned cross-modal associations was observed in delay activity, indicating that this type of representation need not be purely visual. Furthermore, in this same animal, delay activity in one cross-modal task, an auditory-to-visual task, predicted correct and incorrect responses. These results suggest that neurons in inferotemporal cortex contribute to abstract memory representations that can be activated by input from other sensory modalities, but these representations are specific to visual behaviors.
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Modulation of spinal nociceptive transmission by neurotensin microinjected in the rostral ventromedial medulla (RVM) was examined in anesthetized, paralyzed rats. Forty-three spinal dorsal horn neurons in the L3-L5 spinal segments responding to mechanical and noxious thermal stimulation (50 degrees C) of the plantar surface of the ipsilateral hind foot were studied. Spinal units were classified as either wide dynamic range or nociceptive specific and were located in spinal laminae I-V. ⋯ The present series of experiments demonstrate a specific role for neurotensin in the RVM in the modulation of spinal nociceptive transmission, because the peptide was found to both facilitate and inhibit spinal neuron responses to noxious thermal stimulation. Additionally, the facilitatory and inhibitory effects of neurotensin appear to occur via interaction with multiple neurotensin receptors in the RVM that activate independent systems that descend in the ventrolateral funiculi and DLFs, respectively. The results from these experiments are consistent with prior studies demonstrating that the RVM both facilitates and inhibits spinal nociceptive transmission, and they complement previous work showing that neurotensin in the RVM modulates spinal nociceptive behavioral responses.