Clin Lab
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Comparative Study
Precision and comparability of Abuscreen OnLine assays for drugs of abuse screening in urine on Hitachi 917 with other immunochemical tests and with GC/MS.
Abuscreen OnLine assays for drugs of abuse screening in urine have recently been developed for use on Hitachi 917 analyzers (Roche Diagnostics GmbH). The assays are based on the kinetic interaction of microparticles as measured by changes in light transmission. Drug in a sample inhibits the formation of particle aggregates and diminishes absorbance change increases. ⋯ GC/MS was performed for clarification of the discrepant results. In summary, Abuscreen OnLine tests on Hitachi 917 give precise results which compare well when analyzed in different laboratories. They can be rated as convenient and flexible methods for drugs of abuse screening in the routine.
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Comparative Study
Effect of 6-hour exposure to 20 degrees C on the ATP content and other biochemical measures of CPDA-1 packed red cells.
Blood donations for clinical use are routinely stored at 2 degrees C to 6 degrees C for 35 to 42 days. It is common practice for RBCs exposed to temperatures above 10 degrees C to be destroyed, although the American Association of Blood Banks Technical Manual states "Blood exposed to temperatures above 10 degrees C is not necessarily unsuitable for transfusion". To clarify this issue we investigated the effect of 6-hour storage at 20 degrees C on the content of ATP and other biochemical measures of CPDA-1 packed red cells. ⋯ Our data show that a shorter period of moderate warming (6h, 20 degrees C) does not lead to a critical decline of ATP and glucose concentrations in CPDA-1 packed RBCs. The survival of RBCs stored in CPDA-1 is most highly correlated with maintaining ATP concentrations above a value of about 2 mumol per g of Hb [3]. The ATP levels in our study were well above this threshold.