Annals of clinical and laboratory science
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Ann. Clin. Lab. Sci. · Jan 2004
Comparative StudyEffect of protein on hemoglobin and hematocrit assays with a conductivity-based point-of-care testing device: comparison with optical methods.
Point-of-care testing (POCT) for blood hemoglobin and hematocrit (H/H) levels provides rapid patient assessment including the need for transfusion. Conductivity-based methods of blood H/H determinations can be influenced by plasma protein concentration. To assess this factor, we measured H/H levels at varying protein concentrations using two POCT instruments: iSTAT-1 (conductivity method) and Hemocue (optical method). ⋯ The H/H results obtained with the iSTAT-1 instrument gave slightly less correlation with those of the GenS analyzer (r = 0.978 - 0.980) over this protein range. However, the iSTAT-1 results were generally lower than the GenS results, with discrepancies up to 2 g/dL for hemoglobin values and up to 4% for hematocrits at the lowest protein concentration. Therefore, it is recommended that H/H testing in patients with suspected hypoproteinemia or substantial hemodilution should be tested with a non-conductivity-based method.
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Ann. Clin. Lab. Sci. · Jan 2004
Influence of smoking on maternal and neonatal serum malondialdehyde, superoxide dismutase, and glutathione peroxidase levels.
This cohort study investigated postnatal serum malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in 14 active-smoking, 14 passive-smoking, and 15 non-smoking mothers and their newborns on day 7 post-partum. No significant differences were noted among the study groups with respect to MDA (p = 0.63) or SOD levels (p = 0.98) in either the mothers or their infants. ⋯ When GPx activities were analyzed separately in both mothers and infants, a significant difference was noted only between the infants of smoking mothers and the infants of non-smoking mothers (p = 0.015). In conclusion, there was a significant increase in GPx activities of smoking mothers and their infants, suggesting that they may have been exposed to more oxidant stress.