The Tohoku journal of experimental medicine
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Tohoku J. Exp. Med. · Nov 2024
HJURP Derived from Cancer-Associated Fibroblasts Promotes Glutamine Metabolism to Induce Resistance to Doxorubicin in Ovarian Cancer.
Cancer-associated fibroblasts (CAFs) are closely associated with tumor drug resistance. This study intended to delineate how CAFs induced DOX resistance in ovarian cancer. Differential gene expression analysis of ovarian cancer CAFs was completed using Gene Expression Omnibus database. ⋯ HJURP derived from CAFs significantly enhanced viability of A2780-DOX cells and DOX resistance. CAF-derived HJURP fostered glutamine metabolism and mitochondrial TCA cycle in ovarian cancer resistant cells ultimately leading to ovarian cancer DOX resistance. CAF-derived HJURP drove ovarian cancer glutamine metabolism and DOX resistance.
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Tohoku J. Exp. Med. · Nov 2024
Effects of Electrical Stimulation, Pelvic Floor Muscle Exercise, and Biofeedback Program on Improving Pelvic Floor Function and Quality of Life in Postoperative Patients with Early-Stage Cervical Cancer.
Pelvic floor muscle exercise (PME), biofeedback, and electrical stimulation improve pelvic floor function, but the effect of their combination in patients with early-stage cervical cancer is unclear. This study intended to design a combined intervention encompassing these three interventions and explore its effect on pelvic floor function in postoperative patients with early-stage cervical cancer. Totally, 177 postoperative patients with early-stage cervical cancer were assigned to combination (N = 81) and PME (N = 96) groups according to actual interventions. ⋯ Combined intervention (versus PME) was independently related to greater PFDI-20 decline (B = 5.548, P < 0.001) and ICIQ-UI-SF decline (W0-W12) (B = 1.544, P = 0.006). EORTC QLQ-C30 global health status scores at W12 were higher in the combination group versus PME group (P = 0.045), while EORTC QLQ-C30 function and symptom scores at any time points were not different between the two groups (all P > 0.05). Combined intervention achieves greater pelvic floor function improvement and better quality of life compared to PME in postoperative patients with early-stage cervical cancer.
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Tohoku J. Exp. Med. · Nov 2024
Effect of Renal Sympathetic Denervation on Ventricular Electrical Activity in Myocardial Infarction.
Coronary artery blockage causes myocardial infarction (MI), a frequent and serious cardiovascular disease. The early recurrence of post-MI ventricular fibrillation after defibrillation has been widely investigated and treated. This research investigated the relationship between electrophysiological indicators of early recurrence following defibrillation in post-MI ventricular fibrillation and sympathetic renal denervation's therapeutic benefits and probable causes. ⋯ Electrophysiological indications of early recurrence following defibrillation in post-MI ventricular fibrillation are linked to sympathetic renal denervation's therapeutic benefits. Myocardial damage and fibrosis may be reduced, ECG features improved, and the early recurrence rate reduced by sympathetic renal denervation. One possible method of sympathetic renal denervation intervention is reduced sympathetic nerve activity.
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Tohoku J. Exp. Med. · Nov 2024
Shenqi Qiangjing Granules Ameliorate Asthenozoospermia in Mice by Regulating Ferroptosis through the METTL3/GPX4 Signaling Axis.
Asthenozoospermia is a leading cause of male infertility, yet current pharmacotherapies yield suboptimal outcomes, underscoring the urgent need for novel treatment modalities. Herein, we induced asthenozoospermic mouse models using busulfan and investigated the therapeutic effects of Shenqi Qiangjing Granules (SQ) on testicular pathology, serum sex hormone and steroidogenic enzyme levels, and ferroptosis. Furthermore, utilizing GC-1 spg cell lines, we elucidated the role of the METTL3-mediated m6A modification in GPX4 mRNA stability. ⋯ Knockdown of METTL3 mimicked the effects of SQ treatment, while overexpression of METTL3 partially reversed SQ-mediated effects on ferroptosis and asthenozoospermia, underscoring the pivotal role of METTL3 in SQ therapy. In conclusion, the METTL3-GPX4-ferroptosis axis emerges as a novel regulatory pathway in the pathogenesis of asthenozoospermia. Targeting this axis, particularly through interventions such as SQ treatment, holds promise for the management of male infertility.