• E Darendelioglu.
• Bratisl Med J. 2020 Jan 1; 121 (11): 805-811.

AimThe purpose of this research was to examine the suppressing effect of caffeic acid (CA) on colon cancer cells triggered by an overdose of H2O2, and molecular mechanisms involved.MethodThis study examines cell proliferation, measurement of ROS and lipid peroxidation (LPO) levels, total antioxidant status (TAS) level, catalase (CAT) activity, TUNEL assay for calculating the apoptotic index, immunohistochemical staining for caspase-3 proteins, and qRT-PCR for measuring mRNA levels of apoptotic and anti-apoptotic genes.ResultsIn this study, CA considerably suppressed HT-29 cell death induced by cytotoxicity achieved by and overdose of H2O2. Additionally, inducing cells with H2O2 caused a rise in ROS and LPO levels, decrease in TAS level and CAT activity whereas pre-treatment of cells with CA reversed these effects. Additionally, a considerable increase was observed in the expression of Bax, cas-3, cas-8, cyt c, p53 at mRNA levels after H2O2 treatment, however, pre-treatment with CA considerably decreased H2O2-induced upregulation of these genes.ConclusionIn light of all these findings, the antioxidant use should be paid attention to as it could decrease the level of ROS, and in turn decrease the apoptotic cell death which is an unwanted situation in the setting of cancer remedy. Overall, these data revealed that CA can suppress apoptosis in HT-29 cells triggered by an overdose of H2O2 (Fig. 5, Ref. 37).

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