• Bratisl Med J · Jan 2020

    Caffeic acid suppresses HT-29 cell death induced by H2O2 via oxidative stress and apoptosis.

    • E Darendelioglu.
    • Bratisl Med J. 2020 Jan 1; 121 (11): 805-811.

    AimThe purpose of this research was to examine the suppressing effect of caffeic acid (CA) on colon cancer cells triggered by an overdose of H2O2, and molecular mechanisms involved.MethodThis study examines cell proliferation, measurement of ROS and lipid peroxidation (LPO) levels, total antioxidant status (TAS) level, catalase (CAT) activity, TUNEL assay for calculating the apoptotic index, immunohistochemical staining for caspase-3 proteins, and qRT-PCR for measuring mRNA levels of apoptotic and anti-apoptotic genes.ResultsIn this study, CA considerably suppressed HT-29 cell death induced by cytotoxicity achieved by and overdose of H2O2. Additionally, inducing cells with H2O2 caused a rise in ROS and LPO levels, decrease in TAS level and CAT activity whereas pre-treatment of cells with CA reversed these effects. Additionally, a considerable increase was observed in the expression of Bax, cas-3, cas-8, cyt c, p53 at mRNA levels after H2O2 treatment, however, pre-treatment with CA considerably decreased H2O2-induced upregulation of these genes.ConclusionIn light of all these findings, the antioxidant use should be paid attention to as it could decrease the level of ROS, and in turn decrease the apoptotic cell death which is an unwanted situation in the setting of cancer remedy. Overall, these data revealed that CA can suppress apoptosis in HT-29 cells triggered by an overdose of H2O2 (Fig. 5, Ref. 37).

      Pubmed     Full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…