• Arch Med Sci · Jan 2020

    Sulodexide increases mRNA expression of glutathione-related genes in human umbilical endothelial cells exposed to oxygen-glucose deprivation.

    • Bożena Gabryel, Klaudia Bontor, and Tomasz Urbanek.
    • Department of Pharmacology, School of Medicine, Medical University of Silesia, Katowice, Poland.
    • Arch Med Sci. 2020 Jan 1; 16 (6): 1444-1447.

    IntroductionSulodexide (SDX), a heparinoid used to treat vascular diseases, exerts anti-ischemic properties. However, the underlying molecular mechanisms remain unclear. Induction of glutathione (GSH)-dependent genes protects against ischemia. Here, we investigated the effect of SDX on GSH-associated gene expression in human umbilical endothelial cells (HUVECs) using an in vitro ischemia model.MethodsThe transcriptional expression of GSH-related genes (GCLc, xCT, GS, GPx1 and GR) in HUVECs treated without/with SDX (0.5 LRU/ml) under oxygen-glucose deprivation (OGD) condition for 1-6 h was analyzed by real-time polymerase chain reaction.ResultsGCLc and xCT were strongly up-regulated by SDX in HUVECs in the first 2 h of OGD. GS and GPx1 mRNA expression levels were significantly increased during any time interval in ischemic HUVECs treated with SDX. Furthermore, incubation of HUVECs with SDX in OGD for 1-4 h resulted in enhanced expression of GR.ConclusionsOur studies provide the first evidence that SDX activates GSH-related genes in OGD-injured HUVECs.Copyright: © 2019 Termedia & Banach.

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