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- Nirakar Sahoo, Victoria Cuello, Shreya Udawant, Carl Litif, Julie A Mustard, and Megan Keniry.
- Department of Biology, University of Texas - Rio Grande Valley, Edinburg, TX, USA.
- Methods Mol. Biol. 2020 Jan 1; 2115: 365-383.
AbstractCRISPR Cas9 genome editing allows researchers to modify genes in a multitude of ways including to obtain deletions, epitope-tagged loci, and knock-in mutations. Within 6 years of its initial application, CRISPR-Cas9 genome editing has been widely employed, but disadvantages to this method, such as low modification efficiencies and off-target effects, need careful consideration. Obtaining custom donor vectors can also be expensive and time-consuming. This chapter details strategies to overcome barriers to CRISPR-Cas9 genome editing as well as recent developments in employing this technique.
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