• Methods Mol. Biol. · Jan 2020

    CRISPR Guide RNA Design Guidelines for Efficient Genome Editing.

    • Patrick Schindele, Felix Wolter, and Holger Puchta.
    • Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany.
    • Methods Mol. Biol. 2020 Jan 1; 2166: 331-342.

    AbstractThe simple applicability and facile target programming of the CRISPR/Cas9-system abolish the major boundaries of previous genome editing tools, making it the tool of choice for generating site-specific genome alterations. Its versatility and efficacy have been demonstrated in various organisms; however, accurately predicting guide RNA efficiencies remains an organism-independent challenge. Thus, designing optimal guide RNAs is essential to maximize the experimental outcome. Here, we summarize the current knowledge for guide RNA design and highlight discrepancies between different experimental systems.

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