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- Kai Wu and Sami N Malek.
- Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
- Methods Mol. Biol. 2019 Jan 1; 1881: 185-200.
AbstractClustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-based technology enables efficient and precise perturbations of target genomic sites. Combining the endonuclease Cas9 and a pooled guide RNA library allows for systematic screenings of genes associated with a growth disadvantage or lethal phenotype under various conditions in organisms and tissues. Here, we describe a complete protocol for scalable CRISPR/Cas9-based dropout screening for essential genes from focused genomic regions to whole genomes.
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