• Methods Mol. Biol. · Jan 2016

    Resources for Assignment of Phosphorylation Sites on Peptides and Proteins.

    • Vaishnavi Ravikumar, Boris Macek, and Ivan Mijakovic.
    • Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076, Tübingen, Germany.
    • Methods Mol. Biol. 2016 Jan 1; 1355: 293-306.

    AbstractReversible protein phosphorylation is a key regulatory posttranslational modification that plays a significant role in major cellular signaling processes. Phosphorylation events can be systematically identified, quantified, and localized on protein sequence using publicly available bioinformatic tools. Here we present the software tools commonly used by the phosphoproteomics community, discuss their underlying principles of operation, and provide a protocol for large-scale phosphoproteome data analysis using the MaxQuant software suite.

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