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- Hongyan Zhao, Andrew J Creese, and Helen J Cooper.
- School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
- Methods Mol. Biol. 2016 Jan 1; 1355: 241-50.
AbstractHigh-field asymmetric waveform ion mobility spectrometry (FAIMS) is a gas-phase separation technique which, when coupled with liquid chromatography tandem mass spectrometry, offers benefits for analysis of complex proteomics samples such as those encountered in phosphoproteomics experiments. Results from LC-FAIMS-MS/MS are typically complementary, in terms of proteome coverage and isomer identification, to those obtained by use of solution-phase separation methods, such as prefractionation with strong cation-exchange chromatography. Here, we describe the protocol for large-scale phosphorylation analysis by LC-FAIMS-MS/MS.
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