• Methods Mol. Biol. · Jan 2017

    Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS.

    • Mira Jeong, Anna G Guzman, and Margaret A Goodell.
    • Department of Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston, TX, USA.
    • Methods Mol. Biol. 2017 Jan 1; 1633: 137-149.

    AbstractDNA methylation is a major epigenetic modification that regulates gene expression, genome imprinting, and development and has a role in diseases including cancer. There are various methods for whole-genome methylation profiling that differ in cost and resolution. Recent advances in high-throughput sequencing technologies coupled with bisulfite treatment enable absolute DNA methylation quantification and genome-wide single-nucleotide resolution analysis. In this chapter, we provide detailed protocols for whole-genome bisulfite sequencing (WGBS), which captures the complete methylome. Using WGBS, we are able to generate a reference DNA methylome for normal or malignant hematopoietic cells.

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