• Methods Mol. Biol. · Jan 2017

    Navigating the Glycome Space and Connecting the Glycoproteome.

    • Matthew P Campbell, Robyn A Peterson, Elisabeth Gasteiger, Julien Mariethoz, Frederique Lisacek, and Nicolle H Packer.
    • Department of Chemistry and Biomolecular Sciences, Research Drive, Building E8C, Macquarie University, North Ryde, Sydney, 2109, NSW, Australia.
    • Methods Mol. Biol. 2017 Jan 1; 1558: 139-158.

    AbstractUniCarbKB ( http://unicarbkb.org ) is a comprehensive resource for mammalian glycoprotein and annotation data. In particular, the database provides information on the oligosaccharides characterized from a glycoprotein at either the global or site-specific level. This evidence is accumulated from a peer-reviewed and manually curated collection of information on oligosaccharides derived from membrane and secreted glycoproteins purified from biological fluids and/or tissues. This information is further supplemented with experimental method descriptions that summarize important sample preparation and analytical strategies. A new release of UniCarbKB is published every three months, each includes a collection of curated data and improvements to database functionality. In this Chapter, we outline the objectives of UniCarbKB, and describe a selection of step-by-step workflows for navigating the information available. We also provide a short description of web services available and future plans for improving data access. The information presented in this Chapter supplements content available in our knowledgebase including regular updates on interface improvements, new features, and revisions to the database content ( http://confluence.unicarbkb.org ).

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