• Chenglong Liu, Fei Zhang, Haihua Liu, and Fang Wei.
• MD, Associate chief Physician, Department of Anaesthesiology, Gaoyou People's Hospital, Gaoyou Hospital Affiliated, Soochow University, China. Conception and design of the study, manuscript preparation, final approval.
• Acta Cir Bras. 2018 Jul 1; 33 (7): 619-628.

PurposeTo evaluate the role of CX3CL1 and NF-κB in the lumbar disc herniation induced neuropathic pain.MethodsAfter LDH induced by implantation of autologous nucleus pulposus (NP) on the left L5 nerve root was established, mechanical thresholds and thermal hyperalgesia were tested at relevant time points during an observation period of 28 days. Expression of CX3CL1 and NF-κBin the dorsal root ganglion (DRG) were performed by using Western blotting and RT-PCR.ResultsImplantation of autologous nucleus pulposus (NP) induced neuropathic pain, associated with increased mRNA and protein expression of CX3CL1 in the DRG. Moreover, intrathecal injection of neutralizing antibody against CX3CL1 could attenuates LDH-induced persistent pain hypersensitivity. Interestingly, NF-κB activation in the DRGs were found in LDH-induced neuropathic pain. Furthermore, NF-κB downregulation by p65 inhibitor PDTC markedly alleviated LDH-induced mechanical allodynia and thermal hyperalgesia in rat. Importantly, CX3CL1 neutralizing antibody (10 μg/10 μl, i.t.) reduces p-p65 protein level in DRG.ConclusionsCX3XL1 could regulate LDH-induced neuropathic pain through NF-κB pathway. Targeting CX3CL1 and NF-κB may represent a potential treatment for neuropathic pain caused by LDH.

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