• Sertaç Argun Kıvanç, Murat Takım, Merih Kıvanç, and Gülay Güllülü.
• Oltu Public Hospital, Eye Diseases Clinic, Oltu, Erzurum, Turkey.
• Afr Health Sci. 2014 Jun 1; 14 (2): 364-71.

BackgroundIn this study, we attempted to screen and investigate antibacterial activity of Bacillus species, which were isolated from conjunctiva, against other eyes pathogens.MethodsTo examine predominant isolates of Bacillus subtilis, B. pumilus, B. cereus and B. mojevensis, isolated from conjunctiva for their antimicrobial activity against indicator microorganisms as Micrococcus luteus, Staphyloccocus aureus, S. epidermidis, S.hominis, S. lugdunensis, S.warneri, S. haemolyticus, B. cereus, Listeria monocytogenes, and Proteus mirabilis. Growth inhibitions of indicator microorganisms were tested using agar diffusion tests by cells and supernatants of five B. mojevensis, one B. subtilis, four B. cereus and five B. pumilus strains which were isolated from conjunctiva.ResultsThe Bacillus isolates showed variable ability of inhibition against the tested microorganisms. Two strains of B. pumillus, 1 strain of B. subtilis, 5 strains of B. mojevensis, 1 strain of B. cereus were efficacious against the tested microorganisms. Most resistant microorganism to these bacteria was Proteus mirabilis. Two of Gram positive bacteria, S. lugdenensis (K15-9) and S. aureus (SDA48), were also found as resistant.ConclusionsIn this study, Bacillus spp isolated from conjunctiva showed antimicrobial activity against Gram-positive bacteria. Human eye-derived microorganisms and their antimicrobial effects might be a useful source of natural products for the future.

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