• Manal W El-Masry, Heba M Gouda, Iman A Shaheen, Wael Edesa, Naglaa M Hassan, and Rehab Ramzy.
• Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt.
• Afr Health Sci. 2018 Dec 1; 18 (4): 958-964.

BackgroundAcute myeloid leukemia (AML), an aggressive clonal disease, is genetically heterozygous. The prognostic role of expression of Breast Cancer Resistance Protein (BCRP) gene, which behaves as a multidrug transporter, in adult AML is ambiguous.ObjectiveThe objective is to assess the level of mRNA expression of BCRP gene in newly diagnosed cytogenetically normal adult Egyptian AML patients; and to clarify its potential influence and association between therapeutic responsiveness and disease free survival.MethodsThe BCRP gene expression was evaluated by quantifying its mRNA using real time RT-PCR in fifty newly diagnosed cytogenetically normal adult AML patients and 20 healthy normal controls. The expression was evaluated in relation to clinical and prognostic factors, response to treatment and the survival rate.ResultsBCRP mRNA was over expressed in adult AML patients compared to controls. This study showed a positive statistical correlation between BCRP gene expression and the percent of CD34 expression. Statistical analysis did not reveal any association between BCRP expression level and chemotherapeutic responsiveness or disease free survival rate.ConclusionThe significance of BCRP gene expression and its function in AML is very complicated, therefore more standardized clinical studies are needed.

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