• Fawaz Alasmari, Abdullah F Alasmari, Ehab Elzayat, Majed M Alotaibi, Farraj M Alotaibi, Mohamed W Attwa, Fars K Alanazi, Elkhatim H Abdelgadir, Syed Rizwan Ahmad, Faleh Alqahtani, Salim S Al-Rejaie, and Musaad A Alshammari.
• From the Department of Pharmacology and Toxicology (F. Alasmari, F. M. Alotaibi, A. F. Alasmari, Alqahtani, AL-Rejaie, Alshammari); from the Department of Pharmaceutics (Elzayat, Alanazi); from the Department of Pharmaceutical Chemistry (Attwa, Ahmad), College of Pharmacy, King Saud University, and from the Department of Forensic Sciences (M. M. Alotaibi, Abdelgadir), College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Kingdom of Saudi Arabia.
• Saudi Med J. 2022 Jul 1; 43 (7): 678686678-686.

ObjectivesTo detect the cotinine and nicotine serum concentrations of female and male C57BL/6J mice after a 4-week exposure to electronic (e)-cigarette vapors using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).MethodsThis experimental study was carried out at an animal facility and laboratories, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia, between January and August 2020. A 4-week exposure to e-cigarettes was carried out using male and female mice and serum samples were obtained for cotinine and nicotine quantification using UPLC-MS/MS. The chromatographic procedures involved the use of a BEH HSS T3 C18 column (100 mm x 2.1 mm, 1.7 μm) with acetonitrile as a mobile phase and 0.1% formic acid (2:98 v/v).ResultsThe applied methodology has highly efficient properties of detection, estimation, and extraction, where the limit of quantification (LOQ) for nicotine was 0.57 ng/mL and limit of detection (LOD) for nicotine was 0.19 ng/mL, while the LOQ for cotinine was 1.11 ng/mL and LOD for cotinine was 0.38 ng/mL. The correlation coefficient was r2>0.99 for both compounds. The average recovery rate was 101.6±1.33 for nicotine and 100.4±0.54 for cotinine, while the precision and accuracy for cotinine and nicotine were less than 6.1. The serum cotinine level was higher in males (433.7±19.55) than females (362.3±16.27).ConclusionThis study showed that the gender factor might play a crucial role in nicotine metabolism.Copyright: © Saudi Medical Journal.

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