• Feng Xiao, Min Guo, Ruiping Cao, Jue Zou, Lina Yi, and Hongzhu Yan.
• Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China.
• J Coll Physicians Surg Pak. 2023 Feb 1; 33 (2): 149152149-152.

ObjectiveTo determine the function of the YAP-JNK-mitophagy signalling pathway in gastric cancer (GC).Study DesignAn observational study.Place And Duration Of StudySeventh People's Hospital of Shanghai University of TCM (Traditional Chinese Medicine), between June 2019 and June 2021.MethodologyTissues from 30 cases of gastric cancer and corresponding adjacent tissues were collected. RT-qPCR was employed to detect the expression of YAP and JNK in GC samples. MTT, Wound healing and Transwell assays were used to detect changes in GC cell proliferation, migration, and invasion under different stimulation. LC3 immunofluorescence and mitochondrial membrane potential detection were used to analyse the occurrence of mitochondrial autophagy.ResultsThe expression of YAP and JNK were significantly increased in GC tissues (p=0.024, 0.033). YAP knockdown inhibited GC cell proliferation, migration, and invasion. Further studies showed that YAP affects GC cell function by targeting JNK. In addition, YAP-JNK signalling was found to regulate GC cell proliferation, migration, and invasion mainly through regulating the occurrence of mitophagy.ConclusionThese findings revealed that YAP-JNK promotes the development of GC by targeting mitophagy.Key WordsGastric cancer, YAP, JNK, Autophagy.

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