• Neuroscience · May 2023

    Sex and Time: important variables for understanding the impact of constant darkness on behavior, brain, and physiology.

    • Dhyanendra Singh, Abhilash Preetam Ambati, and Palok Aich.
    • School of Biological Sciences, National Institute of Science Education and Research (NISER), HBNI, PO - Bhimpur-Padanpur, Jatni - 752050, Dist. -Khurda, Odisha, India; Centre for Interdisciplinary Science (CIS), National Institute of Science Education and Research (NISER), HBNI, PO - Bhimpur-Padanpur, Jatni - 752050, Dist. -Khurda, Odisha, India; Homi Bhabha National Institute, Training School Complex, Anushaktinagar, Mumbai 400094, India.
    • Neuroscience. 2023 May 21; 519: 738973-89.

    AbstractThe circadian clock can coordinate, regulate and predict physiology and behavior in response to the standard light-dark (LD: 12 h light and 12 h dark) cycle. If we alter the LD cycle by exposing mice to constant darkness (DD: 00 h light and 24 h dark), it can perturb behavior, the brain, and associated physiological parameters. The length of DD exposure and the sex of experimental animals are crucial variables that could alter the impact of DD on the brain, behavior, and physiology, which have not yet been explored. We exposed mice to DD for three and five weeks and studied their impact on (1) behavior, (2) hormones, (3) the prefrontal cortex, and (4) metabolites in male and female mice. We also studied the effect of three weeks of standard light-dark cycle restoration after five weeks of DD on the parameters mentioned above. We found that DD exposure was associated with anxiety-like behavior, increased corticosterone and pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), downregulated neurotrophins (BDNF and NGF), and altered metabolites profile in a duration of DD exposure and sex-dependent manner. Females showed a more robust adaptation than males under DD exposure. Three weeks of restoration was adequate to establish homeostasis in both sexes. To the best of our knowledge, this study is the first of its kind to look at how DD exposure impacts physiology and behavior as a function of sex- and time. These findings would have translational value and may help in establishing sex-specific interventions for addressing DD-related psychological issues.Copyright © 2023 IBRO. Published by Elsevier Ltd. All rights reserved.

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