• Shadia Hamoud Alshahrani, AlmajidiYasir QasimYQBaghdad College of Medical Sciences-department of Pharmacy (Pharmaceutics), Baghdad, Iraq. Electronic address: almajidiyasiir@gmail.com., HasanEkhlas KhammasEKCollege of Pharmacy, Al-Bayan University, Baghdad, Iraq., Musad SalehEbraheem AbduEADepartment of Chemistry, Prince Sattam Bin Abdulaziz University, College of Arts and Science, Wadi Al-Dawasir 11991, Saudi Arabia., Hashem O Alsaab, Ruby Pant, Zahraa F Hassan, Al-HasnawiShaker ShanawaSSCollege of Pharmacy, The Islamic University, 54001 Najaf, Iraq., Romero-ParraRosario MireyaRMUniversidad Continental, Lima, Perú. Electronic address: rosarioromeroparra@gmail.com., and MustafaYasser FakriYFDepartment of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul 41001, Iraq..
• Medical Surgical Nursing Department, King Khalid University, Khamis Mushate, Saudi Arabia.
• Neuroscience. 2023 Sep 1; 527: 526352-63.

AbstractSpinal cord injury (SCI) following trauma is a devastating neurological event that can lead to loss of sensory and motor functions. However, the most effective measures to prevent the spread of damage are treatment measures in the early stages. Currently, we investigated the combined effects of hyperbaric oxygen (HBO) along with epigallocatechin-3-gallate (EGCG) in the recovery of SCI in rats. Ninety male mature Sprague-Dawley rats were randomly planned into five equal groups (n = 18). In addition to sham group that only underwent laminectomy, SCI rats were allocated into 4 groups as follows: control group; HBO group; EGCG group; and HBO + EGCG group. Tissue samples at the lesion site were obtained for stereological, immunohistochemical, biochemical, and molecular evaluation. In addition, behavioral tests were performed to assess of neurological functions. The finding indicated that the stereological parameters, antioxidant factors (CAT, GSH, and SOD), IL-10 gene expression levels and neurological functions were considerably increased in the treatment groups in comparison with control group, and these changes were more obvious in the HBO + EGCG group (P < 0.05). On the other hand, we observed that the density of apoptotic cells and gliosis, the biochemical levels of MDA and the expression levels of inflammatory genes (TNF-α and IL-1β) in the treatment groups, especially the HBO + EGCG group, were considerably reduced in comparison with control group (P < 0.05). We conclude that co-administration of HBO and EGCG has a synergistic neuroprotective effects in animals undergoing SCI.Copyright © 2023 IBRO. Published by Elsevier Ltd. All rights reserved.

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